Occupational Asthma Reference

Marczynski B, Czuppon AB, Hoffarth HP, Marek W, Baur X, DNA damage in human white blood cells after inhalative exposure to methylenediphenyl diisocyanate (MDI)--case report, Toxicol Lett, 1992;60:131-138,

Keywords: DNA, MDI

Known Authors

Xaver Baur, Institute of occupational medicine, Hamburg Xaver Baur

If you would like to become a known author and have your picture displayed along with your papers then please get in touch from the contact page. Known authors can choose to receive emails when their papers receive comments.


A workplace-related inhalative exposure test in a challenge chamber was performed on an industrial worker in a methylenediphenyl diisocyanate (MDI) atmosphere from 5 to 20 ppb and the isolated white blood cell DNA was analysed by electrophoresis, anion-exchange chromatography and melting behaviour. The results of electrophoresis indicate that inhaled MDI induces double-strand breaks of DNA. Some of the DNA fragments were estimated to be in the region of 100-500 bp. Anion-exchange chromatography confirmed this finding. Following denaturation and rapid renaturation the results demonstrated that some DNA fragments are cross-linked by MDI. Comparing the melting curves before and after inhalative exposure in the challenge test chamber, genomic DNA revealed differences in the shape of the melting curve (hyperchromic effect). The results suggest that occupational MDI exposure could be associated with white blood cell DNA damage

Full Text

Full text of this reference not available

Please Log In or Register to add the full text to this reference


Please sign in or register to add your thoughts.

Oasys and occupational asthma smoke logo