Occupational Asthma Reference

Sandiford CP, Nieuwenhuijsen MJ, Tee RD, Taylor AJ, Measurement of airborne proteins involved in Bakers' asthma, Clin Exp Allergy, 1994;24:450-456,

Keywords: oa, am, baker, flour, mill

Known Authors

Rosemary Tee, Royal Brompton Hospital Rosemary Tee

If you would like to become a known author and have your picture displayed along with your papers then please get in touch from the contact page. Known authors can choose to receive emails when their papers receive comments.


We have developed a competitive inhibition immunoassay to measure airborne flour, using purified polyclonal rabbit IgG antibodies to cereal flour antigens. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed different flours contained proteins of molecular weight similar to those present in air sample eluates. Western blotting demonstrated that the purified rabbit IgG antibodies bound to proteins whose molecular weights are similar to human allergens. Air sample eluates obtained from a large bakery had slopes of inhibition parallel to cereal flour when compared in the immuno-inhibition assay. The assay identified airborne proteins from several flours with a detection limit of 1 microgram ml-1. Using this immunoassay we have shown that a wide range of airborne flour concentrations occur in flour mills and a smaller range bakeries. This assay which allows measurement of the concentration of flour aeroallergen in the workplace will be valuable both in epidemiological studies and in examining the effectiveness of interventions to reduce the concentration of flour in the air

Full Text

Full text of this reference not available

Please Log In or Register to add the full text to this reference


Please sign in or register to add your thoughts.

Oasys and occupational asthma smoke logo